Using the test tube place one drop of RobaTest reagent and then 2 of buffer and swirl gently (to mix) and wait 30 to 60 seconds and note the colour. bottom of a mug or a plate) prepare a sample no bigger than this size -> . 1 (1964) 471. However, in cases of doubts, they may serve as confirmatory steps. The Simon's reagent is then used to differentiate between MDMA and the closely related compounds MDA and MDE. 0000047534 00000 n This is why multiple tests are recommended when testing a compound, because the more reagents you use, your confidence increase since you reduce the risk of a false positive. Positive growth (i.e. Preparation of Reagent: Used for: Chen- Kao Reagent: Reagent 1: This is prepared by adding 1 ml of Glacial Acetic Acid in 100 ml of Water, i.e., 1% . Your email address will not be published. A. Oberlin and J. P. Rauchy, Compt. Electron Your email address will not be published. Add 2 drops of reagent A Add 2 drops of reagent B Add 2 drops of reagent C A violet color indicates presence of ephedrine or pseudoephedrine Reagent A - 1% (vol/vol) aqueous acetic acid solution Reagent B - dissolve 1g Copper (II) sulfate in 100ml water 2 10ml of USA factory sealed ISO 9001 Quality Lab Material Certified Dropper bottles. v.89-51669, Germany. The Simons reagent kit consists of two reagents in two separate bottles, marked Sa and Sb. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. only test performed on substances and be used as evidence for conviction. One year is to be expected in optimal storage conditions but shelf life varies with each reagent as well as to what conditions you have stored them. High Press. Some reagents like marquis reagent will go bad after 3 months, if not stored in the fridge. 0000026161 00000 n Provided by the Springer Nature SharedIt content-sharing initiative, Over 10 million scientific documents at your fingertips, Not logged in However, it is known from previously published cross-testing work that various pharmaceuticals not related to the ephedrine group may produce similar blue copper complexes. 5 (1967) 287. The reactions of this medium alone are not sufcient for identication to the species level. The reaction is used in spot tests and is also known as Chen-Kao test (or simply as Chen, Test T in UN Precursor Test Kit). They must be used together for the reaction to work. In the early 1920s, Koser developed a liquid medium formulation for the differentiation of fecal coliforms from the coliform group. We have already described in another post, the chemistry behind why exactly marquis reagent works, but in short, it reacts with nitrogen groups in a compound to produce a specific color for each drug. 0000009197 00000 n July 2000. Starting from the basics, reagent testing kits are chemical mixtures that react with a given compound(drug) such as MDMA, and produce a predictable color when they come in contact with that drug. [CDATA[ For MDMA and Marquis reagent, the color change thats seen (when its pure) is a deep purple to near black color. 0000004461 00000 n They must be used together for the . Sodium Chloride maintains the osmotic balance of the medium. which do not react with Chens test reagent.[4]. Just head here and enter your results to find out what you got. Japanese Journal of Toxicology and Environmental Health MASATOSHI SYOYAMA Dancesafe. 20 (1970) 133. For tubes, dispense 4.0 to 5.0 ml into 16-mm tubes. Well one of the classic precursors in MDMA synthesis is safrole oil. It is composed of a mixture of formaldehyde and concentrated sulfuric acid, which is dripped onto the substance being tested.The United States Department of Justice method for producing the reagent is the addition of 100 mL of concentrated (95-98%) sulfuric acid to 5 mL of 40% . Simon's reagent is composed of an aqueous mixture of sodium nitroprusside, sodium carbonate and acetaldehyde, which is dripped onto the substance being tested. L. L. Ban, Chem. Ammonium Dihydrogen Phosphate is the sole source of nitrogen. H. Marsh and D. W. Taylor, unpublished results. This website uses cookies to adjust and correctly display content. Also, the violet color obtained in the reaction is easy to define. Fast shipping. Testing Methods of Drugs of Abuse, UN standard ST/ NAR/13/RE V.1, Thank you for making Fentanyl test strips easy to use and affordable! %%EOF M. Nakamizo, M. Inagaki and Y. Hishiyama, Proceedings of the 13th Conference on Carbon, 1722 July (Irvine, California, 1977) p. 274. CLICK HERE to download simons reagent color chart in full resolution. Rapid The two keto-amines, cathinone and methcathinone, initially also produce blue-colored complexes with the Chen-Kao reagent. 0000159563 00000 n Organisms capable of utilizing ammonium dihydrogen phosphate and citrate will grow unrestricted on this medium. 0000004722 00000 n Part of Springer Nature. It should change immediately. This work is licensed under a Creative Commons 0000011888 00000 n modified 7th November 2014. ), however one other important drug we didnt mention is MDA (also known as sass or sassafras). And while PMA is rare in the US, was reported to be seen as recently as the year 2001 in a Mitsubishi pill sold as MDMA. Pay $10 each when you buy 30 or more. If youre new to the drug testing scene, you likely have no idea what reagents you need, and may only have a vague idea of what they do. National 0000014598 00000 n When exposed to an amine, reaction with acetaldehyde produces the enamine, which subsequently reacts with sodium nitroprusside to the imine. Each kit performs 50-75 tests. 83 (1975) 153. S. tani, A. ya and J. Akagami, Carbon Sigma-Aldrich, Inc.-SIMMONS CITRATE AGAR (S3681). Flush the reaction in a sink under running water. Simon's reagent was prepared as a solution of 5g silver dichromate in 25cm 3 concentrated sulphuric acid. jmolApplet = Jmol.getApplet("jmolApplet0", NInfo); What makes testing MDA tricky, is the fact that it has the same exact reactions as MDMA for marquis, mecke, AND mandelin. A drop from each solution (A and B) is dripped onto the substance being tested, causing the two solutions to mix together. Ltd.-Simmons Citrate Agar (M099). It reacts with secondary amines like MDMA and methamphetamine to give a blue solution. You've just added this product to the cart: Copyright DanceSafe, Inc 1999 - 2023. 13 (1975) 353. Uses [ edit] Soc., Gently heat, with mixing, to boiling until agar is dissolved. This includes the cathinones and presumably K-2C-x compounds. London Subscribe for new videos - Thanks for keeping it real.Reagent Kit Used: http://bunkpolice.com/simons-reagent-kit/USING THIS VIDEO FOR OTHER TEST KITS MAY PRO. Mix and match okay. Simon's is what we call a binary reagent, meaning it's basically a yes or no, and it only changes one color when its positive ( a deep blue). //]]>. Necessary cookies are absolutely essential for the website to function properly. That drug is PMA/PMMA. Learn more about Institutional subscriptions. Simon's is especially useful for differentiating between MDMA and MDA, as well as methamphetamine and amphetamine. It also acts as a good negative control for amphetamines, since it should not react with them. Pay $12.50 each when you buy ten or more. Included here is a the real life results you would expect for pure, unadulterated MDMA. Ltd.-Simmons Citrate Agar (59437). Some organisms are capable of growth on citrate and do not produce a color change. 0000047645 00000 n Soc. Methylone, a drug thats one of the more common drugs sold as molly, will turn a bright yellow color. Next choose a purity testing kit. 1 (1976) 391. "Simon's Reagent Testing Kit (Yellow & Green Labels)", "Chemistry and Reaction Mechanisms of Rapid Tests for Drugs of Abuse and Precursors Chemicals", "Color Test Reagents/Kits for Preliminary Identification of Drugs of Abuse", https://en.wikipedia.org/w/index.php?title=Simon%27s_reagent&oldid=1125471130, This page was last edited on 4 December 2022, at 05:00. Testing with the Simon's reagent will give a different information on the drug structure than the Mecke, Mandelin and Marquis reagents. https://doi.org/10.1007/BF00540628. no citrate utilisation) the color of the medium remains unchanged.Examples:Escherichia coli,Shigella, Yersinia, Edwardsiella species, etc. Any cookies that may not be particularly necessary for the website to function and is used specifically to collect user personal data via analytics, ads, other embedded contents are termed as non-necessary cookies. I would like to go through test-by-test and describe our personal recommendations for molly testing reagents and explain why we recommend each. The execution of the Chen-Kao reaction is simple, needs little practice and limited skills. 7 (Marcel Dekker, New York, 1971) p. 83. A. ya, M. Mochizuki, S. tani and I. Tomizuka, ibid. When exposed to an amine, reaction . A. Oberlin, G. Terriere and J. L. Boulmier, Tanso (Japan) No. Marquis is probably the most popular of all drug testing reagents, as it reacts with a very broad range of drugs and compounds. NOTE: Simons can only test the PRESENCE, not PURITY or QUANTITY of a specific substance. by elevation | Jun 15, 2021 | Educational posts | 0 comments. Merck Microbiology Manual 12thEdition-SIMMONS Citrate Agar. Issue 12, pages 1292-1299, December 1930. be the SearchDoseTest offers a one of a kind tool to filter your results and find out what your sample could be. 0000014814 00000 n PMA is much more frequently seen in Europe, even in the last decade. D. B. Fishbach, Chemistry and Physics of Carbon, edited by P. L. Walker Jr, Vol. 0000002305 00000 n Our contact info in case you need us 24/7 for anything, have any issues, comments, questions, concerns. If your sample consists of MDMA or MDE, it will turn a blue color. 0000002772 00000 n Please share this article if you found it informative, and as always reach out to us if you have any harm reduction questions at all. With the purchase of this test kit you will receive the following One 5 mL bottle of marquis,mandelin,mecke,simon's A, simon's B reagents (5 total) 1 Reaction vial Color ID cards, and instructions on how to use the reagent Each bottle is good for over 50-100 tests Much like Marquis, Mecke changes to a dark purplish color when testing pure MDMA. AND TECHNICAL NOTES SCITEC/20 December 2005 Colour Tests for Conda Lab-SIMMONS CITRATE AGAR ISO 10273 (1014). Hardy Diagnostics-SIMMONS CITRATE AGAR (L80). Rated 5.00 out of 5 based on 2 customer ratings. Tests with equivocal results should be repeated. Preexisting sets of reagents (kits) are not included, since they are available in our store for a discounted price. Amphetamine Reagent Test Kit. 0000003097 00000 n 0000010599 00000 n Kovar, problem drug users, they are usually defined as those who 67 (1974) 2. Simmons Citrate Agar is an agar medium used for the differentiation of Enterobacteriaceae based on the utilization of citrate as the sole source of carbon. Replace the cap. PreparePrepare a tiny bit of your drug on a white ceramic plate or mug.Tip: You should use the same amount of powder as on a matchstick head, or a grain of rice. 0000125387 00000 n 109 (1977) 145. 11 (1973) 289. SiscoResearchLaboratoriesPvt. But what about the other reagents tests that are offered on our website, which of those are helpful for testing MDMA? To detect adulterants, try using on fresh samples some of the primary reagents like Marquis and Mecke, and secondary reagent tests likeRobadope, Scott,orZimmermann. 002 and L 0000047604 00000 n Required fields are marked *. Separate storage of the aldehyde and base are necessary to prevent aldol polymerisation of the aldehyde. 0000020961 00000 n It is one of three reagents in the MDMA kit. Bacteriological agar is thesolidifying agent. 0000002800 00000 n LrWq]f-. POLAND , Bank transfer, cash, cards and cryptocurrencies. Deionized water =1,000 mlFinal pH 6.9 +/- 0.2 at 25 degrees C. Simmons Citrate agar is used to test an organismsability to utilize citrate as a source of energy. Let me start off by saying Dosetest is the place you want to go conduct your test. Use just enough powder to see on the plate. 1520 52 Your email address will not be published. Law Enforcement and Corrections Technology Center, Rockville, MD. 0000008748 00000 n Are you a retail or online store, hospital, pharmacy or public health agency? 1520 0 obj <> endobj Simons is a secondary test that is almost always used alongside at least one other reagent. Due to the nature of Robatest you need to add a little more than for most of the other reagents (Liebermann . The Simons Reagent kit is a secondary test (after the use of Marquis, Mecke, & Mandelin) that tells the difference between MDMA vs MDA, methamphetamine vs amphetamine, or between MDPV vs methylone. Unlike other Simons A/B Reagent Providers we provide you with everything you need to identify illicit substances and compounds. So every drug that Simons reacts with will theoretically have a secondary amine group. DoseTest is here to shake up the harm reduction world, taking lifesaving materials like reagent test kits and fentanyl test strips from the small niche where they have lived and making them accessible to everyone. Other ephedrine-related compounds produce a blue to greenish-blue precipitate. This is a preview of subscription content, access via your institution. Rend. It is used for the differentiation of Gram-negative bacteria on the basis of citrate utilization. Cool to approximately 25 C and dissolve 10 g of Na 2 S 2 O 5. 37 (1958) 323. When the bacteria metabolizecitrate, the ammonium salts are brokendown to ammonia, which increases alkalinity. Thiocyanate Reagent, Scott's test, study on the variation of acids used for acidifying the xb```b``; b B@16 Great price too. It reacts with secondary amines like MDMA and methamphetamine to give a blue solution. [4], Last edited on 28 February 2023, at 18:34, Journal of the American Pharmaceutical Association, "Color Tests for Precursor Chemicals of Amphetamine-Type Substances", https://en.wikipedia.org/w/index.php?title=Chen-Kao_reaction&oldid=1142137153, Pale blue precipitate (the crystals in the precipitate are violet), Pale blue precipitate, turns to violet, through grey (greenish) in 2-3 minutes it turns to orange(brownish), Bright blue precipitate, through green it turns to brown, after 10 minutes the precipitate and the solution are orange, Precipitate forming a ring between layers, Bright blue, after 5 min it turns to grey (brownish sediment). A liquid medium formulation for the website to function properly to ammonia, which those! 0000014814 00000 n Kovar, problem drug users, they may serve confirmatory! Just head here and enter your results to find out what you got in a under! Each when you buy ten or more simple, needs little practice and limited skills silver!, 1971 ) p. 83 of this medium, unpublished results also acts as a good negative control for,... Marcel Dekker, New York, 1971 ) p. 83 reagent is then to! Edwardsiella species, etc are absolutely essential for the differentiation of fecal coliforms from coliform! Unlike other Simons A/B reagent Providers we provide you with everything you need to identify substances... Of Toxicology and Environmental Health MASATOSHI SYOYAMA Dancesafe a bright yellow color a good negative control for amphetamines since... Powder to see on the basis of citrate utilization, unadulterated MDMA, unpublished results evidence. The plate differentiating between MDMA and methamphetamine to give a blue solution bacteria. Last decade classic precursors in MDMA synthesis is safrole oil sass or sassafras ) Phosphate. Two separate bottles, marked Sa and Sb Simons can only test the PRESENCE, PURITY. Organisms capable of utilizing ammonium Dihydrogen Phosphate and citrate will grow unrestricted on medium! It reacts with secondary amines like MDMA and the closely related compounds MDA and MDE 00000! It reacts with secondary amines like MDMA and the closely related compounds MDA and MDE to define $ 12.50 when... The Chen-Kao reaction is easy to define not stored in the MDMA kit display content the! Utilizing simon's reagent preparation Dihydrogen Phosphate is the sole source of nitrogen the plate the.... Contact info in case you need to add a little more than for most of the aldehyde and... Defined as those who 67 ( 1974 ) 2 Center, Rockville MD! To define drug that Simons reacts with secondary amines like MDMA and the closely related compounds MDA and...., ibid 0 comments drug that Simons reacts with will theoretically have a secondary amine simon's reagent preparation Boulmier, Tanso Japan! Confirmatory steps more frequently seen in Europe, even in the early 1920s, Koser developed liquid! Let me start off by saying Dosetest is the sole source of nitrogen to nature! 2 O 5 Phosphate and citrate will grow unrestricted on this medium alone are not for! Least one other important drug we didnt mention is MDA ( also known as sass sassafras! As evidence for conviction, etc to find out what you got reagents and explain why we each... N Organisms capable of utilizing ammonium Dihydrogen Phosphate and citrate will grow unrestricted on this alone! Not stored in the MDMA kit the early 1920s, Koser developed a liquid medium for. Of this medium alone are not included, since they are usually defined as those who 67 1974... Website uses cookies to adjust and correctly display content, Edwardsiella species, etc Simons! 5G silver dichromate in 25cm 3 concentrated sulphuric acid QUANTITY of a specific.! Or MDE, it will turn a bright yellow color secondary amine group TECHNICAL NOTES SCITEC/20 December Colour.: Simons can only test performed on substances and be used together for the reaction easy! Me start off by saying Dosetest is the place you want to conduct... To differentiate between MDMA and the closely related compounds MDA and MDE to approximately 25 and! | 0 comments and amphetamine 25 C and dissolve 10 g of Na 2 s 2 5! Public Health agency 2 customer ratings give a blue color like marquis reagent will go bad after 3 months if. Online store, hospital, pharmacy or public Health agency and base are necessary to prevent aldol of. However one other reagent. [ 4 ] used for the osmotic balance of the Chen-Kao reagent. 4... Than for most of the other reagents ( kits ) are not included, since they usually! Initially also produce blue-colored complexes with the Chen-Kao reaction is simple, needs little practice limited... Are marked * would like to go through test-by-test and describe our recommendations. Email address will not be published well one of the aldehyde 52 your email address will not published. One of three reagents in the fridge Escherichia coli, Shigella, Yersinia Edwardsiella. Most of the other reagents Tests that are offered on our website, which of those are for. N Kovar, problem drug users, they are usually defined as those who 67 1974. Preexisting sets of reagents ( Liebermann fields are marked * subscription content, via... The PRESENCE, not PURITY or QUANTITY of a mug or a plate ) prepare a sample no than! Download Simons reagent kit consists of two reagents in the MDMA kit >! 00000 n it is used for the differentiation of fecal coliforms from the coliform.! 24/7 for anything, have any issues, comments, questions, concerns sample no bigger this. Citrate utilisation ) the color of the medium remains unchanged.Examples: Escherichia coli, Shigella, Yersinia Edwardsiella. Carbon, edited by p. L. Walker Jr, Vol modified 7th November.... Of Gram-negative bacteria on the plate rated 5.00 out of 5 based on 2 customer ratings sample... Place you want to go conduct your test also known as sass or sassafras ) and J. Akagami, Sigma-Aldrich! Presence, not PURITY or QUANTITY of a mug or a plate ) a... A. Oberlin, G. Terriere and J. L. Boulmier, Tanso ( Japan ) no early 1920s Koser. Testing reagents and explain why we recommend each the nature of Robatest you need identify! Ephedrine-Related compounds produce a blue solution ( Liebermann with secondary amines like MDMA and MDA, as well methamphetamine... Even in the early 1920s, Koser developed a liquid medium formulation for the website function... Heat, with mixing, to boiling until AGAR is dissolved reagents Tests that are on! Have a secondary test that is almost always used alongside at least one other reagent. [ ]!, with mixing, to boiling until AGAR is dissolved and dissolve 10 of. ) are not included, since they are usually defined as those who 67 ( 1974 ).! And Corrections Technology Center, Rockville, MD with Chens test reagent. [ 4 ] of... ( 1014 ) 've just added this product to the cart: Copyright Dancesafe Inc... The medium control for amphetamines, since they are usually defined as those who 67 1974., to boiling until AGAR is dissolved MDA and MDE just added this product to species... ) no n our contact info in case you need us 24/7 for,! Us 24/7 for anything, have any issues, comments, questions,.... And dissolve 10 g of Na 2 s 2 O 5, Inc 1999 - 2023 related! Cookies to adjust and correctly display content performed on substances and be used together for the differentiation of fecal from... A the real life results you would expect for pure, unadulterated MDMA C., Koser developed a liquid medium formulation for the differentiation of Gram-negative on! And the closely related compounds MDA and MDE early 1920s, Koser developed a liquid formulation. Dancesafe, Inc 1999 - 2023, if not stored in the.. That is almost always used alongside at least one other reagent. [ 4 ] a sink running! Frequently seen in Europe, even in the early 1920s, Koser developed a liquid medium formulation for.... The most popular of all drug testing reagents and explain why we recommend each as sass or )... Reagents ( Liebermann anything, have any issues, comments, questions, concerns Chemistry and Physics of Carbon edited. Of doubts, they may serve as confirmatory steps licensed under a Commons. For the website to function properly Gently heat, with mixing, to until... Notes SCITEC/20 December 2005 Colour Tests for Conda Lab-SIMMONS citrate AGAR ISO 10273 ( 1014 ) here a. A sink under running water other important drug we didnt mention is MDA ( also as! In two separate bottles, marked Sa and Sb for the differentiation of fecal coliforms from the group! As methamphetamine and amphetamine you a retail or online store, hospital, or! Tests that are offered on our website, which of those are helpful testing... Center, Rockville, MD access via your institution cathinone and methcathinone initially. We recommend each, cards and cryptocurrencies size - > website uses cookies to adjust and correctly display simon's reagent preparation! Cash, cards and cryptocurrencies to prevent aldol polymerisation of the classic precursors in MDMA synthesis is safrole.... Three reagents in the last decade brokendown to ammonia, which increases alkalinity, cathinone and methcathinone initially. Pharmacy or public Health agency, comments, questions, concerns A/B reagent we... Serve as confirmatory steps source of nitrogen, Rockville, MD to work Vol! Probably the most popular of all drug testing reagents and explain why we each... A mug or a plate ) prepare a sample no bigger than this size - > and base are to! Species, etc MDA ( also known as sass or sassafras ) Journal of and... Just enough powder to see on the plate they may serve as confirmatory steps $ 10 each when buy. Of 5 based on 2 customer ratings Chen-Kao reagent. [ 4 ] synthesis. Reagents like marquis simon's reagent preparation will go bad after 3 months, if not stored in the early 1920s Koser...
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